| School Name | Grades | Address | Zip | Phone | County | Type | District Name |
| A Plus Learning Academy | K-12 | 810 SW Faith Road
Lake City, FL | 32024 | (386) 438-5495 | Columbia | private | |
| Challenge Learning Center | 6-12 | 1301 NW Labonte Ln
Lake City, FL | 32055 | (386) 755-8296 | Columbia | public | Columbia County Public Schools |
| Columbia City Elementary School | PK-5 | 7438 SW State Rd 47
Lake City, FL | 32024 | (386) 758-4850 | Columbia | public | Columbia County Public Schools |
| Columbia High School | 9-12 | 469 SE Fighting Tiger Dr
Lake City, FL | 32025 | (386) 755-8080 | Columbia | public | Columbia County Public Schools |
| Columbia Virtual School Franchise | 6-12 | 372 W Duval St
Lake City, FL | 32055 | (352) 758-4935 | Columbia | public | Columbia County Public Schools |
| Covenant Community School | 1-8 | 2019 SW Main Blvd
Lake City, FL | 32025 | (386) 755-3636 | Columbia | private | |
| Eastside Elementary School | PK-5 | 256 SE Beech St
Lake City, FL | 32025 | (386) 755-8220 | Columbia | public | Columbia County Public Schools |
| Epiphany Catholic School | 9-12 | 1937 SW Epiphany Ct
Lake City, FL | 32025 | (386) 752-2320 | Columbia | private | |
| Five Points Elementary School | PK-5 | 303 NW Johnson St
Lake City, FL | 32055 | (386) 755-8230 | Columbia | public | Columbia County Public Schools |
| Florida Virtual School | 1-12 | 372 W. Duval St
Lake City, FL | 32055 | (386) 755-8020 | Columbia | public | Columbia County Public Schools |
| Joy Explosion Christian Academy | 4-9, 11 | 3134 SW Pinemount Rd
Lake City, FL | 32024 | (386) 752-6627 | Columbia | private | |
| Lake City Christian Academy | PK-12 | 3035 SW Pinemount Rd
Lake City, FL | 32024 | (386) 758-0055 | Columbia | private | |
| Lake City Middle School | 6-8 | 843 SW Arlington Blvd
Lake City, FL | 32025 | (386) 758-4800 | Columbia | public | Columbia County Public Schools |
| Melrose Park Elementary School | PK-5 | 820 SE Putnam St
Lake City, FL | 32025 | (386) 755-8260 | Columbia | public | Columbia County Public Schools |
| New Generation School | 1-12 | 159 SW Spencer Court
Lake City, FL | 32024 | (386) 758-4710 | Columbia | private | |
| Niblack Elementary School | PK-5 | 837 NE Broadway Ave
Lake City, FL | 32055 | (386) 755-8200 | Columbia | public | Columbia County Public Schools |
| Pinemount Elementary School | PK-5 | 324 SW Gabriel Rd
Lake City, FL | 32024 | (386) 755-8179 | Columbia | public | Columbia County Public Schools |
| Residential Services | 10-12 | 444 W Duval St
Lake City, FL | 32055 | (386) 755-8049 | Columbia | public | Columbia County Public Schools |
| Richardson Middle School | 6-8 | 646 SE Pennsylvania St
Lake City, FL | 32025 | (386) 755-8130 | Columbia | public | Columbia County Public Schools |
| Summers Elementary School | PK-5 | 1388 SW McFarlane Ave
Lake City, FL | 32025 | (386) 755-8250 | Columbia | public | Columbia County Public Schools |
| Tabernacle Baptist Academy | K-11 | 144 SE Montrose Ave
Lake City, FL | 32025 | (386) 752-4274 | Columbia | private | |
| The Blake School of Lake City | PK-12 | 7443 Hwy 90 W
Lake City, FL | 32055 | (386) 752-8874 | Columbia | private | |
| Westside Elementary School | PK-5 | 1956 SW County Rd 252b
Lake City, FL | 32024 | (386) 755-8280 | Columbia | public | Columbia County Public Schools |
