| "Crosspointe Academy, Inc." | 02 - 12 | Private | |
| Acceleration Middle Charter School | 06 - 08 | Alternative Education | |
| Adult Education | AE - AE | Regular | |
| Allen Park Elementary School | PK - 05 | Regular | 10 |
| Alternative Learning Center Central | 06 - 12 | Alternative Education | 1 |
| Apostolic Revival Center Christian School | PK - 06 | Private | |
| Bishop Verot Catholic High School | 09 - 12 | Private | |
| Buckingham Exceptional Student Center | PK - 12 | Special Education | |
| Canterbury School | PK - KG | Private | |
| City of Palms Charter High School | 09 - 12 | Alternative Education | 1 |
| Colonial Elementary School | PK - 05 | Regular | 2 |
| Coronado High School | 09 - 12 | Alternative Education | 1 |
| County Wide Exceptional Child Programs | PK - 12 | Special Education | 2 |
| Creative Minds Montessori School | PK - KG | Private | |
| Crestwell School (Crestwell Higher Learning) | PK - 11 | Private | |
| Crown of Life Christian Academy | PK - 01 | Private | |
| Cypress Lake High School | 09 - 12 | Regular | 5 |
| Cypress Lake Middle School | 06 - 08 | Regular | 9 |
| DE Lasalle Academy of Fort Myers | 09 - 12 | Private | |
| DJB Technical Academy | 09 - 12 | Alternative Education | 6 |
| Dunbar Community School | AE - AE | Regular | |
| Dunbar High School | 09 - 12 | Regular | 4 |
| Early Childhood Learning Services | PK - PK | Regular | 2 |
| Edgewood Academy | PK - 05 | Regular | 3 |
| Edison Park Creative and Expressive Arts School | PK - 05 | Regular | 7 |
| Evangelical Christian School | PK - 12 | Private | |
| First Base Christian Academy | PK - KG | Private | |
| Florida Southwestern Collegiate High School | 09 - 12 | Regular | 10 |
| Fort Myers High School | 09 - 12 | Regular | 9 |
| Fort Myers Institute of Technology | 09 - 12 | Vocational Education | |
| Fort Myers Middle Academy | 06 - 08 | Regular | 3 |
| Fort Myers Preparatory and Fitness Academy | KG - 08 | Regular | 2 |
| Franklin Park Elementary School | PK - 05 | Regular | 2 |
| FT Myers Christian School | PK - 08 | Private | |
| Gateway Charter Elementary School | KG - 04 | Regular | 5 |
| Gateway Charter High School | 09 - 12 | Regular | 5 |
| Gateway Charter Intermediate School | 05 - 08 | Regular | 7 |
| Gateway Elementary School | PK - 05 | Regular | 8 |
| Goodwill Life Academy | 06 - 12 | Special Education | |
| Grace Community School of FT Myers | PK - 04 | Private | |
| Heights Elementary School | PK - 05 | Regular | 8 |
| Hibernian Private School | 08 - 12 | Private | |
| Island Park High School | 09 - 12 | Alternative Education | |
| James Stephens International Academy | PK - 08 | Regular | 2 |
| Lee Adolescent Mothers Program | 06 - 12 | Alternative Education | 3 |
| Lee Charter Academy | KG - 08 | Regular | 4 |
| Lee County ALC Central-High | 09 - 12 | Alternative Education | 2 |
| Lee County Jail | 07 - 12 | Alternative Education | |
| Lee County Virtual Franchise | 06 - 12 | Regular | 8 |
| Lee County Virtual Instruction Program (District Provided) | KG - 05 | Regular | |
| Lee Virtual Instruction (Course Offerings) | 06 - 12 | Regular | |
| Lee Virtual Instruction Program | KG - 12 | Regular | |
| Lexington Middle School | 06 - 08 | Regular | 8 |
| Lily Academy | 03 - 09 | Private | |
| Maimonides Hebrew Day School | PK - 06 | Private | |
| Manatee Elementary School | PK - 05 | Regular | 3 |
| Montessori School of Fort Myers | PK - KG | Private | |
| Oak Hammock Middle School | 06 - 08 | Regular | 4 |
| Orange River Elementary School | PK - 05 | Regular | 3 |
| Orangewood Elementary School | PK - 05 | Regular | 6 |
| Pace School for Girls | 06 - 12 | Alternative Education | |
| Paul Laurence Dunbar Middle School | 06 - 08 | Regular | 6 |
| Peace by Piece Learning Center | PK - 09 | Private | |
| Pivot Charter School | 06 - 12 | Regular | 3 |
| Price Halfway House | 06 - 12 | Alternative Education | |
| Ray V. Pottorf Elementary School | PK - 05 | Regular | 3 |
| Rayma C. Page Elementary School | PK - 05 | Regular | 9 |
| Renaissance School | PK - 06 | Private | |
| Riverdale High School | 09 - 12 | Regular | 5 |
| Royal Palm Exceptional School Center | PK - 12 | Special Education | 1 |
| San Carlos Park Elementary School | PK - 05 | Regular | 5 |
| Six Mile Charter Academy | KG - 08 | Regular | 6 |
| Sonshine Christian Academy | PK - 12 | Private | |
| South Fort Myers High School | 09 - 12 | Regular | 4 |
| Southwest Florida Juvenile Detention Center | 06 - 12 | Alternative Education | |
| Southwest Florida Public Service Academy | AE - AE | Vocational Education | |
| St. Francis Xavier School | PK - 08 | Private | |
| St. Michael Lutheran School | PK - 08 | Private | |
| Summit Christian School | PK - 08 | Private | |
| SW FL Addiction Services-the Vince Smith Center | 06 - 12 | Alternative Education | |
| Tanglewood Elementary School | PK - 05 | Regular | 8 |
| Three Oaks Elementary School | PK - 05 | Regular | 10 |
| Three Oaks Middle School | 06 - 08 | Regular | 8 |
| TICE Elementary School | PK - 05 | Regular | 4 |
| Treeline Elementary School | PK - 05 | Regular | 5 |
| Villas Elementary School | PK - 05 | Regular | 7 |